Radisch S., Poltorak M.P., Wagner M., Cletiu V., Radisch C., Treise I., Pann S., Weigt A., Artner S., Dreher S., Fechner F., Borjan B., Fraessle S.P., Effenberger M., Benke E., Navratil G., Hentschel N., Busch D.H., Schmidt T.G.M., Stemberger C. & Germeroth L. (2022) Next generation automated traceless cell chromatography platform for GMP‑compliant cell isolation and activation. Scientific Reports 12, 6572. > read paper

Schmidt, T.G.M., Eichinger, A., Schneider, M., Bonet, L., Carl, U., Karthaus, D., Theobald, I., Skerra, A. (2021). The role of changing loop conformations in streptavidin versions engineered for high-affinity binding of the Strep-tag II peptide. J. Mol. Biol. 433, 166893. > read paper

Poltorak, M.P., Graef, P., Tschulik, C., Wagner, M., Cletiu, V., Dreher, S., Borjan, B., Fräßle, S.P., Effenberger, M., Turk, M., Busch, D.H., Plitzko, J., Kugler, D.G., Ragan, S., Schmidt, T.G.M., Stemberger, C. & Germeroth L. (2020). Expamers: a new technology to control T cell activation. Scientific Reports 10, 17832. > read paper

Yeliseev, A., Zoubak, L., Schmidt, T.G.M. (2016). Application of Strep-Tactin XT for Affinity Purification of Twin-Strep-Tagged CB2, a G Protein-Coupled Cannabinoid Receptor. Protein Expr. Purif. 131, 109-118

Stemberger, C., Dreher, S., Tschulik, C., Piossek, C., Bet, J., Yamamoto, T.N., Schiemann, M., Neuenhahn, M., Martin, K., Schlapschy, M., Skerra, A., Schmidt, T.G.M., Edinger, M., Riddell, S.R., Germeroth, L., Busch, D.H. (2012). Novel Serial Positive Enrichment Technology Enables Clinical Multiparameter Cell Sorting. PLoS ONE 7, 1-11. > read paper

Schmidt, T.G.M. & Skerra, A. (2007). The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins. Nature Protocols 2, 1528-1535. > read paper

Neudorfer, J., Schmidt, B., Huster, K.M., Anderl, F., Schiemann, M., Holzapfel, G., Schmidt, T.G.M., Germeroth, L., Wagner, H., Peschel, C., Busch, D.H., Bernhard, H. (2007). Reversible HLA multimers (Streptamers) for the isolation of human cytotoxic T lymphocytes functionally active against tumor- and virus-derived antigens. J. Immunological Methods 320, 119-131.

Junttila, M., Saarinen, S., Schmidt, T.G.M., Kast, J., Westermarck, J. (2005). Single-step Strep-tag purification for the isolation and identification of protein complexes from mammalian cells. Proteomics 5, 1199-1203.

Gerdes, W., Kiem, K.-P., Schmidt, T.G.M. (1998). Affinitätsreinigung rekombinanter Proteine mittels Strep-tag, StrepTactin und HPLC. BIOspektrum 1/98, 73-74.

Schmidt, T.G.M., Koepke, J., Frank, R., Skerra, A. (1996). Molecular interaction between the Strep-tag affinity peptide and its cognate target, streptavidin. J. Mol. Biol. 255, 753-766.

Dübel, S., Breitling, F., Kontermann, R., Schmidt, T.G.M., Skerra, A., Little, M. (1995). Bifunctional and multimeric complexes of streptavidin fused to single chain antibodies (scFv). J. Immunological Methods 178, 201-209.

Schmidt, T. G. M. & Skerra, A (1994). One-step purification of bacterially produced proteins by means of the „Strep tag“ and immobilized recombinant core streptavidin. J. Chromatogr. A 676, 337-345.

Schmidt, T.G.M. & Skerra, A. (1993). The random peptide library-assisted engineering of a C-terminal affinity peptide, useful for the detection and purification of a functional Ig Fv fragment. Protein Engineering 6, 109-122.

Glockshuber, R., Schmidt, T.G.M., Plückthun, A. (1992). The disulfide bonds in antibody variable domains: Effects on stability, folding in vitro, and functional expression in Escherichia coli. Biochemistry 31, 1270-1279.